S.NEST®2.0

Real-time metabolic
monitoring and superior
cell culture

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Trusted by 100+ scientists

from Leading Biopharmaceutical Companies and Academic Institutions

At the smallest scale,
for the highest throughput

S.NEST® 2.0 is a culturing system that monitors cell metabolism in real-time and provides effective mixing in both 96 and 24-well plates. By integrating monitoring, mixing, and incubation into a single instrument, S.NEST significantly reduces time and costs associated with cell culture R&D and manufacturing.

Cell metabolic
monitoring

Bioreactor-like
mixing
conditions

CO2 Incubation

Cell metabolic
monitoring

Bioreactor-like
mixing
conditions

CO2 Incubation

Frontal view of S.NEST 2.0
Frontal view of S.NEST 2.0
Frontal view of S.NEST 2.0
Frontal view of S.NEST 2.0
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01. “In-Well” Cell Metabolic Monitoring

↘︎ Mitochondrial respiration and glycolysis insights
↘︎ Real-time, non-invasive, sampling-free monitoring
↘︎ Measurements at 10-min intervals

S.NEST monitors real time DO (dissolved oxygen) and pH in all wells using optical sensors in 96/24-well plates . The data is captured during the entire cell culture period. Our software can further calculate OCR (oxygen consumption rate) and ECAR (extracellular acidification rate) from this data, improving sensitivity to detect metabolic changes earlier.

Application and Tech note

  • 24-well plate format monitoring data
  • 96-well plate format monitoring data

02. Patented “In-Well”
Mixing

✨ Suspension boost:

specially designed vigorous mixing pattern for higher cell density and viability

↘︎ Homogeneous nutrient distribution

↘︎ Higher oxygen transfer rate

↘︎ 3D growing space

↘︎ Increased cell suspension

See your cells thrive with our unique mixing system – efficient pneumatic mixing in 96/24 well plates.

Application and Tech note

  • Suspension boost data
  • Oxygen mass transfer coefficient kLa

03. Independent
Incubation Chambers

Tailor your culture conditions in each of the four independent incubation chambers equipped with precise temperature and CO2 control, humidity monitoring, water reservoir, and UV sterilization. Cultivate flexibly and precisely with independent mixing control for each chamber, allowing parallel execution of both mixing and static culture.

Application and Tech note

  • Chamber environment SQP

DO/pH And OCR/ECAR For Cell Metabolic Analysis

Comprehensive insights into cellular function, adaptation, and responses to various physiological
and pathological conditions.

pH monitoring

Lactate accumulate & Metabolic
switch

↘︎ Feed and pH control time point

Conditions

  • Cell line: mAb-CHO-K1​
  • Medium: CD04​
  • Plate format: 24-well
  • Culture volume: 1,400 uL/well
  • Mixing rate: 10s/cycle
  • Duration: 3 days

DO monitoring

Lactate accumulate & Metabolic
switch

↘︎ Feed and pH control time point

Conditions

  • Cell line: mAb-CHO-K1​
  • Medium: CD04​
  • Plate format: 24-well
  • Culture volume: 1,400 uL/well
  • Mixing rate: 10s/cycle
  • Duration: 3 days

Software interface

Streamlined Interface,
Data at Your Fingertips!

Navigate effortlessly through our user-friendly software interface, allowing for convenient control of the four incubation chambers. Real-time data on mixing status, culture environment, DO, pH, OCR, and ECAR values provides a comprehensive view of your experiment.

Workstation

Offering advanced features with user-centric design, connect multiple systems and manage them all with simple controls in the software. Run parallel experiment without any interference.

Applications

Streamlined cell line development

Early-stage cell clone selection

Miniaturize model for process development

High-throughput drug screening

Analysis of cell metabolism

Higher viability for primary cultures

CHO cell

HEK cell

Hybridoma cell

Stem cell

Cancer cell

iPSC

PBMC

T cell

Spheroid

Organoid

E. coli

Pichia pastoris

Book a demo to try it out
Contact us to view more data

Automation-friendly

Designed for maximum productivity, S.NEST is automation-friendly, saving you both time and labor. Its seamless integration with your robotic arm, along with SiLA 2.0 compatibility, makes it a versatile solution. Build your automated station for a streamlined workflow.

Learn more about S.NEST® 2.0

Brochure
Flyer
Video
App Note
Tech Note

Technical
Specifications

General characteristics

Description Value Unit
Dimensions
Width 785 mm
Depth 435 mm
Height 296 mm
Weight 49 kg
Electrical characteristics
Input voltage 100-240 Vac
Input voltage net tolerance ±10 %
Input voltage frequency 50/60 Hz
Max. input current 11.8-5.4 A
Power mains supply voltage fluctuations ±10 A
Installation category CAT ll -
Input fuse type 250VAC, 16A, time-lag -

Basic configuration

Description Value Unit
Incubation
Temperature control range RT+5 - 45±0.2 °C
CO2 level control range 1-20±0.3 %
Humidity monitoring range 0-100±5(at 37°C) %
Culture
Mixing rate (24-well plate) 10-50s±5 %
Mixing rate (96-well plate) 10-50s±5 %
Working volume (24-well plate) 1,000-1,600 μL
Working volume (96-well plate) 150-200 μL
Working volume (384-well plate) 25-80 μL
Sensing
DO measurement range 0-100±5** %
pH measurement range 6-8±0.2 -
Sampling rate ≥10 min
* Each value above is specified with one standard deviation from its mean (M±1SD)
** Once the reference DO (defined as 100%) has been set, the DO readout may vary between 100 +/- 10% due to the fluctuation of oxygen levels in ambient air.

Ready to Experience the Future of Cell Culture?

Book a demo now to witness the transformative power of S.NEST® 2.0 in action. Accelerate your cell line development, streamline your processes, and unlock new possibilities in cell culture research.
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