High throughput cell selection and expansion the most effective redeveloped immune cells
as well as boosting cell therapy production
Cell therapy in which the T-cells are modified in the laboratory so they can fight tumor cells. T-cells can be taken and isolated from a normal or patient’s blood then modified to express the specific antigen which calls chimeric antigen receptor T-cell (CAR-T).
After large scale of CAR-T cells are grown then injected or implanted into a patient for treatment. c.bird can introduce the procedure of T-cell expansion and accelerate the treatment timelines.
Cell therapy in which the T-cells are modified in the laboratory so they can fight tumor cells. T-cells can be taken and isolated from a normal or patient’s blood then modified to express the specific antigen which calls chimeric antigen receptor T-cell (CAR-T).
After large scale of CAR-T cells are grown then injected or implanted into a patient for treatment. c.bird can introduce the procedure of T-cell expansion and accelerate the treatment timelines.
The Jurkat cell line, an immortalized line of human T lymphocyte cells, was the model used in this study. These data show the C.BIRD culture method can improve human T-cell growth without compromising cell viability in both 96-well and 24-well systems.
Figure 2. The cell growth and doubling time of 96-well C.BIRD and static culture methods in 7 days of continuous culture. A) Viable cell density, B) viability and C) doubling time of day 6. Statistics were performed by unpaired t test. Data are shown as mean ± SEM.
Figure 3. The cell growth and doubling time of 24-well C.BIRD and static culture methods in 7 days of continuous culture. A) Viable cell density, B) viability and C) doubling time of day 6. Statistics were performed by unpaired T test. Data are shown as mean ± SEM.
T cell activation is an important procedure in the development or manufacturing process of T cell-based cell therapies. This study showed that the C.BIRD culture method augmented the activation level of the human T cell line in a 96-well culture scale. This feature can integrate with another C.BIRD function, improving human T cells growth. These features can help researchers shorten the timeline for process development of T cell-based cell therapies by using the C.BIRD culture method.
Figure 2. (A) Bright-field microscopy images of formed clusters by Jurkat cells cultured with CD3/CD28 Dynabeads™ at indicated ratios under static and C.BIRD cultures. Scale bars = 250 µm. (B) Expression level of IL-2 in each culture group. Significance of P value is listed as the following: 0.05 (ns), <0.05 (*), <0.01 (**), <0.001 (***) and <0.0001 (****).
CYTENA BPS is a part of BICO. Our products aim to reduce the organ shortage and speed up drug development by providing accessible life science solutions that combine biology and technology, fundamentally shifting the global healthcare industry.
CYTENA BPS is a part of BICO.Our products aim to reduce the organ shortage and speed up drug development by providing accessible life science solutions that combine biology and technology, fundamentally shifting the global healthcare industry.
Cookie | Duration | Description |
---|---|---|
cookielawinfo-checbox-analytics | 11 months | This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Analytics". |
cookielawinfo-checbox-functional | 11 months | The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". |
cookielawinfo-checbox-others | 11 months | This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other. |
cookielawinfo-checkbox-necessary | 11 months | This cookie is set by GDPR Cookie Consent plugin. The cookies is used to store the user consent for the cookies in the category "Necessary". |
cookielawinfo-checkbox-performance | 11 months | This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Performance". |
viewed_cookie_policy | 11 months | The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. It does not store any personal data. |