Cell therapy & stem cell

High throughput cell selection and expansion the most effective redeveloped immune cells

as well as boosting cell therapy production

Introduction

Cell therapy in which the T-cells are modified in the laboratory so they can fight tumor cells. T-cells can be taken and isolated from a normal or patient’s blood then modified to express the specific antigen which calls chimeric antigen receptor T-cell (CAR-T).

After large scale of CAR-T cells are grown then injected or implanted into a patient for treatment. c.bird can introduce the procedure of T-cell expansion and accelerate the treatment timelines.

Introduction

Cell therapy in which the T-cells are modified in the laboratory so they can fight tumor cells. T-cells can be taken and isolated from a normal or patient’s blood then modified to express the specific antigen which calls chimeric antigen receptor T-cell (CAR-T).

After large scale of CAR-T cells are grown then injected or implanted into a patient for treatment. c.bird can introduce the procedure of T-cell expansion and accelerate the treatment timelines.

CAR T-CELL THERAPY

Stem cell

PERFORMANCE DATA

AN INNOVATIVE CELL CULTURE FOR HUMAN T CELLS

The Jurkat cell line, an immortalized line of human T lymphocyte cells, was the model used in this study. These data show the C.BIRD culture method can improve human T-cell growth without compromising cell viability in both 96-well and 24-well systems.

Figure 2. The cell growth and doubling time of 96-well C.BIRD and static culture methods in 7 days of continuous culture. A) Viable cell density, B) viability and C) doubling time of day 6. Statistics were performed by unpaired t test. Data are shown as mean ± SEM.

Figure 3. The cell growth and doubling time of 24-well C.BIRD and static culture methods in 7 days of continuous culture. A) Viable cell density, B) viability and C) doubling time of day 6. Statistics were performed by unpaired T test. Data are shown as mean ± SEM.

Augment the Human T Cell Activation in Standard 96-well Plates Using the C.BIRD Microbioreactor

T cell activation is an important procedure in the development or manufacturing process of T cell-based cell therapies. This study showed that the C.BIRD culture method augmented the activation level of the human T cell line in a 96-well culture scale. This feature can integrate with another C.BIRD function, improving human T cells growth. These features can help researchers shorten the timeline for process development of T cell-based cell therapies by using the C.BIRD culture method.

Figure 2. (A) Bright-field microscopy images of formed clusters by Jurkat cells cultured with CD3/CD28 Dynabeads™ at indicated ratios under static and C.BIRD cultures. Scale bars = 250 µm. (B) Expression level of IL-2 in each culture group. Significance of P value is listed as the following: 0.05 (ns), <0.05 (*), <0.01 (**), <0.001 (***) and <0.0001 (****).

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