Innovative lid for your
standard microplate
Our patented lid is the core technology that enables the reciprocating “in-well” mixing for 96/24-well plates.
One cell culture kit comes with
1 x patented lid and 1 x standard well plate.
S/C.NEST cell culture kit
C.BIRD cell culture kit
The principle of our innovative lid
Precision-engineered for optimal cell culture
Optimized Airflow
Our lid’s airflow port ensures a continuous exchange of positive and negative air pressure. It enables mixing and maintains the ideal oxygen levels for a healthier cell culture, while minimizing the evaporation rate.
NEST lid kLa: well-defined oxygen transfer rate: 24-well plate
The volumetric oxygen mass transfer coefficient, kLa, plays a pivotal role in cell culture. It is the rate of oxygen transfer from gas to liquid and profoundly impacts cell viability and productivity. Here we present the well-defined kLa for your optimal experimental design.
NEST lid
evaporation
performance
system is balanced by…
system is balanced by…
Water reservoir for humidity maintenance
Patented lid to evenly distribute the evaporation rate across all wells
Minimal and homogenous
evaporation after 7 days of
culturing in S/C.NEST
Accurate planning with our
evaporation estimation guide
Over 90% humidity sustained
by integrated water reservoir
Minimal and homogenous
evaporation after 7 days of
culturing in S/C.NEST
Minimal and homogenous evaporation after 3 days of culturing in S/C.NEST
Minimal and homogenous evaporation
after 3 days of culturing in S/C.NEST
| Condition | |
| Medium | PBS |
| Mixing Rate | 10 Seconds/Cycle |
| Culture Volume | 1400μL |
| Water Reservoir Volume | 40mL DI Water |
| Duration | End Point Measurements On Day 3 |
| Number Of Replicates | 3 |
Minimal and homogenous evaporation after 3 days of culturing in S/C.NEST
| Condition | |
| Medium | PBS |
| Mixing Rate | 10 Seconds/Cycle |
| Culture Volume | 200μL |
| Water Reservoir Volume | 40mL DI Water |
| Duration | End Point Measurements On Day 3 |
| Number Of Replicates | 3 |
Accurate planning with our
evaporation estimation guide
Accurate planning with our evaporation estimation guide
Accurate planning with our evaporation estimation guide
Plan your experiments with more precision. Evaporation in S/C.NEST comes in two forms: mixing-induced, where the liquid evaporates during movement, and manipulation-induced, where loss occurs when the lid is uncovered. Our evaporation estimation guide helps you anticipate liquid loss based on your experimental protocols.
Total Evaporation ≈ 20.9 (μL/day/well)× Mixing duration + 15.0 (μL/time/well) × Uncovering times
| Condition | |
| Plate format | 24-well plate |
| Medium | 10 PBS |
| Mixing rate | 10 seconds/cycle |
| Culture volume | 1400μL |
| Water reservoir volume | 50mL DI water |
| Duration | end point measurements on day 7 |
| Number of replicates | 3 |
Over 90% humidity sustained
by integrated water reservoir
Over 90% humidity sustained by integrated water reservoir
Over 90% humidity sustained by integrated water reservoir
Experience optimal chamber humidity. Our integrated water reservoir allows the humidity to be maintained by adding DI water.
Each of the data points below represents one 7-day evaporation experiment, conducted with different settings in different instruments at different sites. As the maximum evaporated water volume observed is 30.6 mL and the minimal volume for covering the water reservoir surface is 10mL, we recommend adding 40mL of water to the reservoir for a 7-day culture to maintain chamber humidity above 90% at all times!
| Condition | |
| Medium | PBS |
| Culture Volume | 1400μL |
| Duration | End Point Measurements On Day 3 |
Ordering Information
| S.NEST Consumables | ||
| Product No. | Product Name | Description |
| D16110024309 | S.NEST Cell Culture Kit - Greiner, 24 Well, 10 Sets |
|
| CBS161101026 | S.NEST Cell Culture Kit - Corning, 96 Well, Combination Pack, 10 Sets (5 DO and 5 pH) |
|
| CBS161101027 | S.NEST Cell Culture Kit - Corning, 96 Well, DO, 10 Sets |
|
| CBS161101028 | S.NEST Cell Culture Kit - Corning, 96 Well, pH, 10 Sets |
|
| C.NEST Consumables | ||
| Product No. | Product Name | Description |
| D16110025167 | C.NEST Cell Culture Kit - Greiner, 24 Well, 20 Sets |
|
| D16110022683 | C.BIRD Cell Culture Kit – Eppendorf, 96 Well, 20 Sets |
|
| C.BIRD Consumables | ||
| Product No. | Product Name | Description |
| D16110024715 | C.BIRD Cell Culture Kit – Corning, 96 Well, 20 Sets |
|
| D16110022683 | C.BIRD Cell Culture Kit – Eppendorf, 96 Well, 20 Sets |
|
| D16110022684 | C.BIRD Cell Culture Kit – Greiner, 24 Well, 20 Sets |
|
